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% Generated by roxygen2 (4.1.1): do not edit by hand
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% Please edit documentation in R/normalize.and.transform.R
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\name{normalize.ODs}
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\alias{normalize.ODs}
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\title{Normalize OD readings for an entire array of well objects}
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\usage{
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normalize.ODs(well.array, normalize.method = "default", blank.value = NULL,
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start.index = 2, add.constant = 1)
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}
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\arguments{
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\item{well.array}{an array of well objects.}
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\item{normalize.method}{see Details}
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\item{blank.value}{user can enter a blank OD measurement for uninoculated wells. if NULL, defaults to the value of the first OD measurement of each well.}
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\item{start.index}{which timepoint should be used as the first one after inoculation (defaults to the 2th one)}
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\item{add.constant}{add a numeric constant to all timepoints in all wells.}
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}
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\description{
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Normalize OD readings for an entire array of well objects
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}
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\details{
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Note: This function does not write any new OD values to the well objects in the array - it only
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fills the "norm" slot of each well object in the array with a value that will be subtracted
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from all OD measurements when returning data from the wells using the function <data.from> (see well.class.R)
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These functions make use of <raw.data> which simply returns the raw time and OD of a well (also see well.class.R)
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note this is the only normalization function that acts on an entire array instead of an individual well.
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normalize.method settings:
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\describe{
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\item{default}{subtracts the blank OD (either specified by <blank.value> or taken from the first timepoint as default)
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of each well from all timepoints in that well}
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\item{average.blank}{subtracts the mean of all first OD timepoints on a plate from all timepoints in all wells on that plate}
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\item{average.first}{takes the mean of the difference between the OD of the specified <start> timepoint and the first timepoint of all wells on a plate
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and subtracts this value from all timepoints in all wells on that plate}
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\item{anything else}{do nothing}
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}
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}
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