% Generated by roxygen2 (4.1.1): do not edit by hand % Please edit documentation in R/normalize.and.transform.R \name{transform.ODs} \alias{transform.ODs} \title{Log-transform OD readings for a single well object} \usage{ \method{transform}{ODs}(input.well, use.log = T, blank.value = NULL, start.index = 2, negative.OD.cutoff = 10, constant.added = 1, ...) } \arguments{ \item{input.well}{an object of class well} \item{use.log}{gets added to the "use.log" slot of the well object. this will determine whether the log-transformed data or raw normalized data is returned using the function \code{data.from}.} \item{blank.value}{user can enter a blank OD measurement for uninoculated wells. if NULL, defaults to the value of the first OD measurement of each well.} \item{start.index}{which timepoint should be used as the first one after inoculation (defaults to the 2th one)} \item{negative.OD.cutoff}{if any ODs below the specified blank value are detected before this index timepoint, the entire well is discarded.} \item{constant.added}{similar to added.constant.} \item{...}{Additional arguments for this function.} } \description{ This function adds a "log.OD" column to the "screen.data" slot of a well object with log-transformed data. The raw data is kept intact. It also checks to see if any of the raw OD values (before a certain timepoint) is below the blank OD. This can be disastrous for the log(OD) transform. }