You can not select more than 25 topics
Topics must start with a letter or number, can include dashes ('-') and can be up to 35 characters long.
33 lines
1.4 KiB
33 lines
1.4 KiB
% Generated by roxygen2 (4.1.1): do not edit by hand
|
|
% Please edit documentation in R/normalize.and.transform.R
|
|
\name{transform.ODs}
|
|
\alias{transform.ODs}
|
|
\title{Log-transform OD readings for a single well object}
|
|
\usage{
|
|
\method{transform}{ODs}(input.well, use.log = T, blank.value = NULL,
|
|
start.index = 2, negative.OD.cutoff = 10, constant.added = 1, ...)
|
|
}
|
|
\arguments{
|
|
\item{input.well}{an object of class well}
|
|
|
|
\item{use.log}{gets added to the "use.log" slot of the well object. this will determine whether the log-transformed data
|
|
or raw normalized data is returned using the function \code{data.from}.}
|
|
|
|
\item{blank.value}{user can enter a blank OD measurement for uninoculated wells. if NULL, defaults to the value of the first OD measurement of each well.}
|
|
|
|
\item{start.index}{which timepoint should be used as the first one after inoculation (defaults to the 2th one)}
|
|
|
|
\item{negative.OD.cutoff}{if any ODs below the specified blank value are detected before this index timepoint, the entire well is discarded.}
|
|
|
|
\item{constant.added}{similar to added.constant.}
|
|
|
|
\item{...}{Additional arguments for this function.}
|
|
}
|
|
\description{
|
|
This function adds a "log.OD" column to the "screen.data" slot of a well object with log-transformed data.
|
|
The raw data is kept intact.
|
|
It also checks to see if any of the raw OD values (before a certain timepoint) is below the blank OD.
|
|
This can be disastrous for the log(OD) transform.
|
|
}
|
|
|